Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene.
Identifieur interne : 004023 ( Main/Exploration ); précédent : 004022; suivant : 004024Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene.
Auteurs : Safiyh Taghavi [États-Unis] ; Tanja Barac ; Bill Greenberg ; Brigitte Borremans ; Jaco Vangronsveld ; Daniel Van Der LelieSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 2005.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Burkholderia cepacia (génétique), Burkholderia cepacia (métabolisme), Dépollution biologique de l'environnement (MeSH), Feuilles de plante (microbiologie), Microbiologie du sol (MeSH), Populus (croissance et développement), Populus (microbiologie), Racines de plante (microbiologie), Réaction de polymérisation en chaîne (MeSH), Tiges de plante (microbiologie), Toluène (pharmacocinétique), Transfert horizontal de gène (MeSH).
- MESH :
- croissance et développement : Populus.
- génétique : Burkholderia cepacia.
- microbiologie : Feuilles de plante, Populus, Racines de plante, Tiges de plante.
- métabolisme : Burkholderia cepacia.
- pharmacocinétique : Toluène.
- Amorces ADN, Dépollution biologique de l'environnement, Microbiologie du sol, Réaction de polymérisation en chaîne, Transfert horizontal de gène.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Burkholderia cepacia (genetics), Burkholderia cepacia (metabolism), DNA Primers (MeSH), Gene Transfer, Horizontal (MeSH), Plant Leaves (microbiology), Plant Roots (microbiology), Plant Stems (microbiology), Polymerase Chain Reaction (MeSH), Populus (growth & development), Populus (microbiology), Soil Microbiology (MeSH), Toluene (pharmacokinetics).
- MESH :
- chemical , pharmacokinetics : Toluene.
- chemical : DNA Primers.
- genetics : Burkholderia cepacia.
- growth & development : Populus.
- metabolism : Burkholderia cepacia.
- microbiology : Plant Leaves, Plant Roots, Plant Stems, Populus.
- Biodegradation, Environmental, Gene Transfer, Horizontal, Polymerase Chain Reaction, Soil Microbiology.
Abstract
Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Noninoculated plants or plants inoculated with the soil bacterium B. cepacia Bu61(pTOM-Bu61) were used as controls. Inoculation of poplar had a positive effect on plant growth in the presence of toluene and reduced the amount of toluene released via evapotranspiration. These effects were more dramatic for VM1468, the endophytic strain, than for Bu61. Remarkably, none of the strains became established at detectable levels in the endophytic community, but there was horizontal gene transfer of pTOM-Bu61 to different members of the endogenous endophytic community, both in the presence and in the absence of toluene. This work is the first report of in planta horizontal gene transfer among plant-associated endophytic bacteria and demonstrates that such transfer could be used to change natural endophytic microbial communities in order to improve the remediation of environmental insults.
DOI: 10.1128/AEM.71.12.8500-8505.2005
PubMed: 16332840
PubMed Central: PMC1317371
Affiliations:
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Greenberg</name></author><author><name sortKey="Borremans, Brigitte" sort="Borremans, Brigitte" uniqKey="Borremans B" first="Brigitte" last="Borremans">Brigitte Borremans</name></author><author><name sortKey="Vangronsveld, Jaco" sort="Vangronsveld, Jaco" uniqKey="Vangronsveld J" first="Jaco" last="Vangronsveld">Jaco Vangronsveld</name></author><author><name sortKey="Van Der Lelie, Daniel" sort="Van Der Lelie, Daniel" uniqKey="Van Der Lelie D" first="Daniel" last="Van Der Lelie">Daniel Van Der Lelie</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16332840</idno><idno type="pmid">16332840</idno><idno type="doi">10.1128/AEM.71.12.8500-8505.2005</idno><idno type="pmc">PMC1317371</idno><idno type="wicri:Area/Main/Corpus">003F14</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003F14</idno><idno type="wicri:Area/Main/Curation">003F14</idno><idno 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(MeSH)</term><term>Burkholderia cepacia (genetics)</term><term>Burkholderia cepacia (metabolism)</term><term>DNA Primers (MeSH)</term><term>Gene Transfer, Horizontal (MeSH)</term><term>Plant Leaves (microbiology)</term><term>Plant Roots (microbiology)</term><term>Plant Stems (microbiology)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (growth & development)</term><term>Populus (microbiology)</term><term>Soil Microbiology (MeSH)</term><term>Toluene (pharmacokinetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Burkholderia cepacia (génétique)</term><term>Burkholderia cepacia (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Feuilles de plante (microbiologie)</term><term>Microbiologie du sol (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (microbiologie)</term><term>Racines de plante (microbiologie)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Tiges de plante (microbiologie)</term><term>Toluène (pharmacocinétique)</term><term>Transfert horizontal de gène (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Toluene</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Burkholderia cepacia</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Burkholderia cepacia</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Burkholderia cepacia</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de 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xml:lang="en">Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Noninoculated plants or plants inoculated with the soil bacterium B. cepacia Bu61(pTOM-Bu61) were used as controls. Inoculation of poplar had a positive effect on plant growth in the presence of toluene and reduced the amount of toluene released via evapotranspiration. These effects were more dramatic for VM1468, the endophytic strain, than for Bu61. Remarkably, none of the strains became established at detectable levels in the endophytic community, but there was horizontal gene transfer of pTOM-Bu61 to different members of the endogenous endophytic community, both in the presence and in the absence of toluene. This work is the first report of in planta horizontal gene transfer among plant-associated endophytic bacteria and demonstrates that such transfer could be used to change natural endophytic microbial communities in order to improve the remediation of environmental insults.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16332840</PMID><DateCompleted><Year>2006</Year><Month>02</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>71</Volume><Issue>12</Issue><PubDate><Year>2005</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene.</ArticleTitle><Pagination><MedlinePgn>8500-5</MedlinePgn></Pagination><Abstract><AbstractText>Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Noninoculated plants or plants inoculated with the soil bacterium B. cepacia Bu61(pTOM-Bu61) were used as controls. Inoculation of poplar had a positive effect on plant growth in the presence of toluene and reduced the amount of toluene released via evapotranspiration. These effects were more dramatic for VM1468, the endophytic strain, than for Bu61. Remarkably, none of the strains became established at detectable levels in the endophytic community, but there was horizontal gene transfer of pTOM-Bu61 to different members of the endogenous endophytic community, both in the presence and in the absence of toluene. This work is the first report of in planta horizontal gene transfer among plant-associated endophytic bacteria and demonstrates that such transfer could be used to change natural endophytic microbial communities in order to improve the remediation of environmental insults.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Taghavi</LastName><ForeName>Safiyh</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Brookhaven National Laboratory, Biology Department, Building 463, Upton, NY 11973-5000. vdlelied@bnl.gov.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barac</LastName><ForeName>Tanja</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Greenberg</LastName><ForeName>Bill</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Borremans</LastName><ForeName>Brigitte</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Vangronsveld</LastName><ForeName>Jaco</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>van der Lelie</LastName><ForeName>Daniel</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>3FPU23BG52</RegistryNumber><NameOfSubstance UI="D014050">Toluene</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" 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uniqKey="Vangronsveld J" first="Jaco" last="Vangronsveld">Jaco Vangronsveld</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Taghavi, Safiyh" sort="Taghavi, Safiyh" uniqKey="Taghavi S" first="Safiyh" last="Taghavi">Safiyh Taghavi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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